研究方向
真核复杂类型细胞如干细胞/免疫细胞等基因转录后调控的机制研究正越来越受到研究者的关注。相比转录水平（组蛋白修饰，DNA修饰，转录因子等），转录后调控（RNA加工，稳定性调控等）对于细胞来说，它消耗能量更少，更便于细胞精细调控，满足复杂细胞应对发育分化/抗炎应急等外来信号的需求。本研究组围绕近期snoRNA可调控mRNA3'末端加工而影响mRNA表达水平这一新的功能发现，开展以下问题的科学探索。
1.snoRNA调控mRNA3'末端加工的机制
2.除了参与经典的rRNA/snRNA的甲基化/假尿嘧啶修饰的功能，snoRNA是否会组装到非经典的蛋白复合物，而会有其他的功能？
3. snoRNA是否能通过调控靶mRNA水平而参与干细胞分化的命运决定?
教育和工作经历
2014/12-至今，中山大学，中山医，副教授
2011/6-2014/12
美国加州大学欧文分校，分子生物学，博士后
2009/6-2011/5
美国加州大学圣地亚哥分校，分子生物学，博士后
2003/9-2009/5
中科院生化细胞所，分子生物学，博士
1999/9-2003/6
南开大学，生物科学，本科
主持或参加科研项目及人才计划项目情况
广州市协同创新项目70万 2018-2021 参与
国家青年自然面上基金24万 2016.1-2018.12 主持
中山大学百人计划启动基金 200万 2015.1-2018.1 主持
中山大学重大项目培育和新兴交叉学科资助计划项目 20万 2015.10-2017.10 主持
研究生和人才培养
3名硕士生在读，其中2名获得2017年度国家奖学金
发表文章记录
1.Shi,Junjie;Huang, Chunliu;Huang,Shanshan; Yao Chengguo* (2018) snoRNAs associated with mRNA 3' processing complex: new wine in old bottle. RNA Biology (Invited point-of-view Article)
2.Huang, Chunliu; Shi, Junjie; Guo, Yibin; Huang, Weijun; Huang, Shanshan; Ming, Siqi; Wu, Xingui; Zhang, Rui; Ding, Junjun; Zhao, Wei; Jia, Jie; Huang, Xi; Xiang, Peng; Shi, Yongsheng*; Yao, Chengguo* (2017) A snoRNA modulates mRNA 3’ end processing and regulates the expression of a subset of mRNAs. Nucleic Acids Research 2017 (NAR Breakthrough Article) (*corresponding authors)
3.Maliheh Movassat, Tara L. Crabb, Anke Busch, Chengguo Yao, Derrick J. Reynolds, Yongsheng Shi & Klemens J. Hertel (2016) Coupling between alternative polyadenylation and alternative splicing is limited to terminal introns. RNA Biology. DOI:10.1080/15476286.2016.1191727
4.Serena L. Chan, Ina Huppertz, Chenguo Yao, James J. Moresco, John R. Yates III, Jernej Ule, Yongsheng Shi (2014) CPSF30 and Wdr33 directly bind to AAUAAA in mammalian mRNA 3’ processing. Genes & Dev. 28(21):2370-80.
5.Brad Lackford *, Chengguo Yao*, Georgette M. Charles *, Lingjie Weng, Xiaofeng Zheng, Eun-A Choi, Xiaohui Xie, Ji Wan, Yi Xing, Johannes M, Freudenberg, Raja Jothi, Guang Hu,Yongsheng Shi (*contributed equally). Fip1 regulates mRNA alternative polyadenylation to promote stem cell selfrenewal. EMBO J. 2014 16;33(8):878-89.
6.Chengguo Yao, Lingjie Weng, Yongsheng Shi. Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq. Methods Mol Biol. 2014, 1126: 399-410.
7.Chengguo Yao, Yongsheng Shi. Global RNA polyadenylation mapping by PAS-seq. Methods Mol Biol. 2014, 1125:179-85.
8.Li Wang, Yi-liang Miao, Xiaofeng Zheng, Brad Lackford, Bingying Zhou, Leng Han, Chengguo Yao, James Ward, Adam Burkholder, Inna Lipchina, David C. Fargo, Konrad Hochedlinger, Yongsheng Shi, Carmen J. Williams, and Guang Hu. The THO Complex Regulates Pluripotency Gene mRNA Export and Controls Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming. Cell Stem Cell. 2013 13(6):676-90.
9.Chengguo Yao, Eun-A Choi, Lingjie Weng, Xiaohui Xie, Ji Wan, Yi Xing, James J. Moresco, Patricia G. Tu, John R. Yates, Yongsheng Shi. Overlapping and distinct functions of CstF64 and CstF64t in mammalian mRNA 3’ processing. RNA. 2013 19(12):1781-90.
10.Chengguo Yao, Jacob Biesinger, Ji Wan, Lingjie Weng, Yi Xing, Xiaohui Xie, Yongsheng Shi. Transcriptome-wide analyses of CstF64–RNA interactions in global regulation of mRNA alternative polyadenylation. Proc Natl Acad Sci U S A. 2012, 109 (46): 18773-8.
11.Chengguo Yao, Janina E. Moretti, Peter E. Struss, Junaid A. Spall, Ulrich F. Muller. Arginine Cofactors on the Polymerase Ribozyme. PLoS One. 2011; 6(9): e25030.
12.Chengguo Yao, Ulrich F Muller. Polymerase Ribozyme efficiency increased by G/T-rich DNA oligonucleotides. RNA. 2011, 17(7):1274-81.
